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Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h

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Among these, metabolic reprogramming is not merely a byproduct of tumorigenesis but a selective adaptation that supports tumor growth and progression [73,74,75]

Scientifica 2025(1):6637793 Yousefi R, Saidpour A, Mottaghi A (2019) The effects of Spirulina supplementation on metabolic syndrome components, its liver manifestation and related inflammatory markers: a systematic review