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Separation was performed using an waters symmetry C18 chromatographic column (5 m*250 mm*4.6 mm), with a 25 min gradient program of 0.05 mol/L aqueous solution of CH3COONa containing 1% DMF (pH=6.5) (A) and acetonitrile (B): 16% B (1 min), 1631% B (4 min), 3136% B (9.5 min), 3655% B (11 min), 5560% B (12 min), 60100% B (15 min), 100% B (20 min), 10016% B (25 min)
Set reminders for yourself well before the expiration date so you have plenty of time to complete all the necessary steps
I'm not questioning others, I can not smell it on my wrist
