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Starting at 0.25mg weekly for the first four weeks, then increasing to 0.5mg for weeks 5-8, then to 1mg for weeks 9-12, and continuing to step up through 1.7mg and 2.4mg at four-week intervals

In addition, Bid cleavage and the translocation of both Bax and cleaved Bid to mitochondria are involved in the early outer mitochondrial membrane permeabilization after an APAP overdose [4952]

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OsDHAR was purified by Ni-NTA affinity chromatography [5 mL HisTrap FF column (GE Healthcare, Little Chalfont, UK) on an AKTA FPLC system] and eluted with a step gradient of 250 mM imidazole