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The role of glutathione S-transferase GliG in gliotoxin biosynthesis in Aspergillus fumigatus

Then, 10 l aliquots of these reactions were directly added to 90 l ultrafiltered flavan-3,3,4-triol ( 4 ) to initiate the second step of the two-step in vitro assays with 3,4- cis -leucocyanidin ( 6 ) as described below

The ability to quantify the extent of glutathionylation would represent a substantial improvement in this approach, but would require double labelling using two different labels for the protein of interest and the BioGEE and as such poses significant technical challenges

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