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The absence of IGF-1 pathway activation is also consistently observed across experimental systems, reinforcing its value in studies requiring separation of metabolic and growth-related signaling pathways

4.9 Western blotting Six hours after seeding (or 24 h after doxycycline induction for IMR-5/75), the cells were treated with either MitoQ IC50, DPI IC50, Vin IC50, (M+D)/2, (M+D+V)/3 or vehicle and incubated at 37C for 24 h

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