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Cyanocobalamin B12 injection 1000 mcg is used to treat and prevent vitamin B12 deficiency, which can lead to anemia and neurological issues

The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 g/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 g/mL) (Bio-Sciences) for 30 min protected from light, at room temperature

In some instances, applying a cold compress may relieve the pain

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