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The tCho signal is comprised mainly of glycerophosphocholine and phosphocholine [13]

Each sample (8 l) was injected onto an Agilent Poroshell 120 EC-C18 (100 2.1 mm, 2.7 um) in a Shimadzu Nexera UHPLC system at a flowrate of 0.2 ml/min using a gradient elution created between buffer A (10 mM tributylamine aqueous solution adjusted pH to 4.95 with 15 mM acetic acid) and buffer B of methanol, from 10% to 40% (buffer B) in 30 min at room temperature

Glutathione and mitochondria, 2014 Mitochondrial ROS generation and antioxidant defense systems

Among these enzymes, GPX4 is the principal endogenous suppressor of ferroptosis by limiting lipid peroxidation within biological membranes (Fig