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The increase of fluorescence due to GSH conjugate was converted to the corresponding concentration of GSH in the reaction mixture since 1.0 fluorescence unit recorded on the chart had been calibrated to correspond to 0.1 M concentration of GSH conjugate

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The rats were then placed in clean wire-bottom cages (no food or water was supplied) and euthanized via CO 2 inhalation 15 minutes after the oral gavage