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In this study, we describe a novel methodology for the determination of 19 biomarkers of health effects: malondialdehyde (MDA), 8-isoprostaglandin-F 2 (8-PGF 2 ), 11--prostaglandin-F 2 (11-PGF 2 ), 15-prostaglandin-F 2 (15-PGF 2 ), 8-iso-15-prostaglandin-F 2 (8,15-PGF 2 ), 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-HdG), 8-hydroxyguanine (8-HG), dityrosine (diY), allantoin (Alla), and two metabolic products of 4-hydroxynonenal (HNE), namely 4-hydroxy-2-nonenal glutathione (HNE-GSH) and 4-hydroxy-2-nonenal mercapturic acid (HNE-MA) (in total, 12 oxidative stress biomarkers, OSBs)

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