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Initially, senescence was confirmed through SA--gal staining following treatment with 4 M of CDDP (Figure

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78 The NLRP3 inflammasome, part of the nucleotide-binding domain and leucine-rich repeat protein family complex, is essential in the development of numerous infections and inflammatory disorders

Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate