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RNA isolation and quantitative reverse transcription-polymerase chain reaction Total RNA was isolated using the ISOGEN reagent (Nippon Gene) and reverse transcribed to cDNA using the ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan)

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[DOI] [PubMed] [Google Scholar] Garcia-Ruiz C, Fernndez-Checa JC

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