In addition, when mTORC1-S6K1 is activated, S6K1 can phosphorylate eIF4B at Ser422 and promote the formation of the mRNA translation initiation complex
For expression assays, a positive colony for each construct in each strain was grown in LB medium that was supplemented with 100 g/ml ampicillin and 50 g/ml chloramphenicol overnight at 37 C with constant stirring at 200 rpm
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After 30 min, wells were aspirated, washed with PBS and plates were sealed with adhesive foil (VWR, 60941-124)
