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Therefore, we collected EVs from dimethyl sulfoxide (DMSO), estradiol, and progesterone-treated BUTE-CM (6 pellets per biological replicate) and analyzed their metabolic content by non-targeted metabolomics

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A matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) assay revealed several proteins with antioxidant functions including a GST that were differentially expressed between compatible and incompatible interactions, indicating the differential accumulation of ROS in infected tissues (Li et al., Hemibiotrophic Fungi The important role of GSTs in antifungal plant resistance was demonstrated also in hemibiotrophic plant-fungus interactions