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Cells were washed with phosphate buffered saline solution (PBS) and detached from culture flask using a mild cell detachment buffer Accutase to protect the extracellular portion of the cell membrane proteins

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During the acute phase of the illness, all patients had been admitted to one of the official quarantine facilities in Al-Najaf city specialized in the treatment of acute COVID-19, including Al-Sader Medical City of Najaf, Al-Hakeem General Hospital, Al-Zahraa Teaching Hospital for Maternity and Pediatrics, Imam Sajjad Hospital, Hassan Halos Al-Hatmy Hospital for Transmitted Diseases, Middle Euphrates Center for Cancer, and Al-Najaf