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Moreover, studies based on gene expression data and tissue-specific genome-scale metabolic models have demonstrated that the gut microbiota can influence host amino acid metabolism and contribute to changes in glutathione metabolism [77]

And in animal experiments, what theyve done is theyve said, Okay, lets give these animals inhibitors of their mitochondria at specific locations and see what methylene blue does. And if you dont have any inhibitors, and if the animal is genetically healthy, then you add methylene blue, they get less ATP, so the mitochondria is less effective at converting food to usable energy

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Samples were brought to 20 mL total volume with a buffer containing 20 mM Tris, 300 mM NaCl, and 0.05%LMNG