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hGPX4-1-F: 5-GATCCGTGAGGCAAGACCGAAGTAAACTCGAGTTTACTTCGGTCTTGCCTCACTTTTTG-3 hGPX4-1-R: 5-AATTCAAAAAGTGAGGCAAGACCGAAGTAAACTCGAGTTTACTTCGGTCTTGCCTCACG-3 hGPX4-2-F: 5-GATCCGGGAGTAACGAAGAGATCAAACTCGAGTTTGATCTCTTCGTTACTCCCTTTTTG-3 hGPX4-2-R: 5-AATTCAAAAAGGGAGTAACGAAGAGATCAAACTCGAGTTTGATCTCTTCGTTACTCCCG-3 hGPX4-3-F: 5-GATCCACGTCAAATTCGATATGTTCACTCGAGTGAACATATCGAATTTGACGTTTTTTG-3 hGPX4-3-R: 5-AATTCAAAAAACGTCAAATTCGATATGTTCACTCGAGTGAACATATCGAATTTGACGTG-3 Determination of intracellular Fe2+ levels For Liperfluo staining (Dojindo, Kumamoto, Japan), Raji cells were seeded in a 48-well plate

doi: 10.7759/cureus.10097 40 LiuJHanPWuJGongJTianD

It also provides 48% of the DV for magnesium

This process can generate harmful substances that may promote inflammation, damage the organ and can contribute to alcoholic fatty liver disease