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Upon activation, mast cells rapidly release pruritogenic mediators such as histamine, prostaglandin D 2 (PGD 2 ), tryptase, and -hexosaminidase (Poto et al., 2022), which collectively stimulate C-type sensory nerve fibers and trigger neuroimmune amplification (Siiskonen and Harvima, 2019)

After lysis, crude cell extracts are processed to remove cellular debris by centrifugation

Inui S, Fukuzato Y, Nakajima T, Kurata S, Itami S (2007) Androgen receptor co-activator Hic-5/ARA55 as a molecular regulator of androgen sensitivity in dermal papilla cells of human hair follicles

Mansilla WD, Marinangeli CPF, Cargo-Froom C, Franczyk A, House JD, Elango R et al