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TSH, FT4, FT3, anti-TPO, and anti-TG were quantified using electrochemiluminescent immunoassays (Roche Diagnostics, Basel, Switzerland), and plasma h-TRAB levels were measured via a radioimmunometric assay (B.R.A.H.M.S, Hennigsdorf, Germany)

Testicular histology was assessed using Hematoxylin-Eosin staining to observe seminiferous tubule morphology, and a Johnsons score was applied to quantify spermatogenic function based on a 10-point scale

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