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The hCMEC/D3 cells (passage 2832) were seeded on collagen coated culture flasks (2.5-3 10 4 /cm 2 ) or glass slides (4 10 4 /cm 2 ) and maintained at 37C with 5% CO 2 exposure in EBM-2 basal medium (Lonza, Walkersville, MD, USA) supplemented with 5% FBS (Atlanta Biologicals, Lawrenceville, GA, USA), chemically defined lipid concentrate (Life Technologies, Carlsbad, CA, USA), growth factors, antibiotic/antimycotic (1:1, Atlanta Biologicals, GA, USA and HEPES (10 mM)
Legislative Impact on Workplace Policies When implementing a workplace smoking policy, employers should be aware of specific areas of concern in regard to restricting tobacco use
Sanitary Design and Operation A production room is straightforward, conceptually: design the space so walls, floors, and ceilings can be washed and sanitized, then verified (ATP swabs) to confirm the cleaning process is effective
again, the band is so aesthetically pleasing
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