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To maintain optimal stability, keep the pen away from light, and do not expose it to repeated temperature changes

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After 2 days in vitro (DIV), the cultures were changed to Prenatal Mouse Neuron Media composed of Neurobasal media (Gibco/Fisher Scientific, Cat# 21-103-049) supplemented with B-27 Plus (Fisher Scientific, Cat# A3582801), GlutaMAX Supplement, and penicillin/streptomycin