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The mechanism for this was related mainly to the effect of fiber on gut microbiota, which tends to regulate the hormones and fight against inflammation [126]

Using 100 oil-immersion and differential interference contrast optics, photomicrographs of each section were collected as a montage with a microscope equipped with a motorized X-Y-Z stage and a digital charge-coupled device video camera (Provis AX70

The clear cell lysate was collected and the proteins in the supernatant were precipitated by treating with 10 % (v/v) trichloroacetic acid (TCA) on ice for 30 min

Lyophilized vs liquid peptide stability Lyophilized (freeze-dried) powder: Most peptides sold in this form Moisture removed via freeze-drying Very stable when dry Can last years if stored properly Requires reconstitution before use Lyophilized stability: Freezer (-20C): 2-5+ years for most peptides Refrigerator (2-8C): 1-2 years typical Room temperature: Weeks to months (varies by peptide) Key factor: Keeping it DRY (moisture is enemy) Liquid (reconstituted) peptides: Peptide dissolved in water Much less stable than powder Water enables hydrolysis Bacterial contamination risk higher Limited shelf life Liquid stability: Refrigerated with bacteriostatic water: 28-30 days typical Refrigerated with sterile water: 5-7 days maximum Room temperature: Hours to 1-2 days only Frozen (reconstituted): DO NOT FREEZE liquid peptides Key principle: Keep peptides as dry powder until needed, then use reconstituted solution within recommended timeframe