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The animals were sacrificed on day 1 of the intermittent fasting period at 9 a.m., and then different brain areas (midbrain, thalamus and hypothalamus, and hippocampus) were quickly excised according to Glowinski and Iversen (1966) using ice plate weighted and stored at 80 C for further investigations

Urbancsok, J., Bones, A

That credential does not authorize them to prescribe a controlled or prescription only substance, to determine the dose of an injectable compound, to teach injection technique as a clinical service, or to manage adverse reactions

The glutathione concentration of unknown sample is measured by measuring the absorbance at 415 nm and comparing it with standard curve for GSSG which is plotted every time new for any glutathione estimation