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Rats with normal hearing and PPI behavior were divided into experimental and control groups

Gene expression analysis was performed via qPCR using the SYBR-Green qPCR Super Mix-UDG kit on a Mastercycler ep realplex detection system (Agilent, USA)

Meanwhile, lipid peroxidation and iron colorimetric assays indicated elevated MDA and Fe 2+ concentrations, and reduced GSH concentration and GSH-Px activity in the placenta of PE patients (Fig