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The keloid tissues for organ culture were firstly incubated in a DMEM containing 10% FBS at 37 C for 24 h, and the medium was replaced by the DMEM containing 2% FBS, the final concentration of 25 M FOXO4-DRI was added in the medium immediately

Amino acid analysis and mass spectrometry were in agreement with the expected structure and within the experimental error of the method (mass spectrometry0.9 amu, amino acidanalysis10%)

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