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Cai L, Zhao C, Cao X, Lu M, Li N, Luo Y, et al

To infect cancer cells with lentivirus, cells were infected twice with culture medium containing 2 mL lentivirus, 200 L FBS and 5 mg/ mL polybrene (Sigma) at 37 C for 24 and 48 h

After blocking in 1% skim milk in Tw-PBS for 1 h at room temperature, the membrane was incubated with SMA (dilution 1:2000

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