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However, as time has gone by and smoking has become a declining social stigma, the receptacle has become the standard DC connector to supply electrical power for portable accessories used in the automobile, so its more likely to be referenced as a "12 V auxiliary power outlet" and may be power limited as a consequence

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After secondary antibody incubation, the slices were washed with physiological saline (three 5 min washes) and then mounted onto SuperFrost Plus Slides, counterstained with Fluoromount-G medium (Southern Biotech) and coverslipped

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